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Premier design is a primary design of complete pairs Prime application.-PCR Primer design for multiplex PCR experiments. Cross enhancer sequences of multiple species detection oligo species. Pathogenic locate very conservatively-regions.

Allele specific-Exclusively amplify a single member of the series of related sequences.-Degenerate-Start with the amino acid sequence, reverse translate and design oligos in areas with high levels of degeneration automatically.-Nested / Check multiplex-homology between multiple candidate oligos. Restriction enzyme analysis and patterns Select from a database of more than 800 enzymes and 200 W alignments-Load sequences aligned motifs. Clustal common after ClustalW alignment using all resources, including EBI.

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